http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114085804-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e37a9c655eb6d0f3b6837eab31f9cf8a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2513-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0698 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5088 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adff837c5791d9702de53cdb768d1448 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8fab2aeccf30c4df414f91b6b0f01d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_379714c1b1360449b4f76d5cf5a19b5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4fe506d12f528ac73db01c5d0708110 |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114085804-A |
| titleOfInvention | Skin organoid formed by in vitro induction and differentiation of human embryonic stem cells and high-efficiency culture method and application thereof |
| abstract | The invention discloses a skin organoid formed by in vitro induction and differentiation of human embryonic stem cells, and a high-efficiency culture method and application thereof, wherein the process comprises the following steps: pretreatment of differentiation; inducing human embryonic stem cells to differentiate by adopting a first differentiation culture medium to form epidermal ectoderm and brain neural crest ectoderm cells; inducing the brain neural crest ectodermal cells obtained in the first step into dermal fibroblasts by adopting a second differentiation culture medium and an E6 culture medium in sequence; and (3) sequentially adopting a third differentiation culture medium and a fourth differentiation culture medium to sequentially induce the development and maturation of the sensory nerve endings and the hair follicles of the cultures obtained in the step two. The invention has the advantages that: the efficiency of differentiating the human embryonic stem cells into the skin organoids in three dimensions is improved, the yield of the skin organoids is 55.30 +/-11.06 percent, and the culture cost is saved; the obtained skin organoid has layered epidermis, dermis, hair follicle, melanocyte and sensory nerve ending, and can be used for disease research such as infectious diseases, hereditary dermatosis and skin tumor, and provides hair source for hair transplantation of alopecia patients. |
| priorityDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.