http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114085785-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_056da8b71b885b52d9d4d24bfcbb1d2f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_359a77dc0dd493d1fe8526ecf3327739 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e075dd7e6c25078cb5ac6b1acf50acb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0cfe2578209169043e0e16e026e69ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9f33e88d9eacd85e5f2ab2c49030e98 |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114085785-A |
| titleOfInvention | Saccharomyces cerevisiae gene engineering bacterium and construction method and application thereof |
| abstract | The invention provides a saccharomyces cerevisiae gene engineering bacterium, which is constructed by replacing a GAL4 promoter in saccharomyces cerevisiae with a copper ion suppression promoter pCTR1 or pCTR3 and replacing a GAL80 promoter with a copper ion induction promoter pCUP1, wherein the engineering bacterium can suppress the expression of pGAL1, pGAL2, pGAL7 and pGAL10 promoters controlling exogenous genes in a GAL gene regulation system under the action of copper ions in a seed culture period, thereby avoiding the premature production of products and obviously improving the passage stability. The GAL gene regulation system of the engineering bacteria is not influenced by the concentration of galactose, the biological expression of the terpenoid is improved, the construction method is simple, the production cost is low, the production of a target product can be realized without adding any inducer in the fermentation production stage, the engineering bacteria are suitable for large-scale production, and the engineering bacteria have great application prospects in the production field of the terpenoid. |
| priorityDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.