http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114085289-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2022-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114085289-B |
| titleOfInvention | Construction method and application of semisynthetic single-domain antibody library |
| abstract | The invention provides a construction method and application of a semi-synthetic single-domain antibody library, and relates to the technical field of antibody engineering. The construction method comprises the steps of constructing a deletion type single domain antibody initial library, amplifying a camel single domain antibody FR1-FR3 region gene fragment by taking the deletion type single domain antibody initial library as a template, designing and artificially synthesizing a section of oligonucleotide sequence with diversity, and splicing the single domain antibody FR1-FR3 region gene fragment and the artificially synthesized fragment into a full-length single domain antibody sequence with diversity, thereby preparing the semisynthetic single domain antibody library. The construction method of the invention obtains a high-capacity and high-diversity single-domain antibody semi-synthetic antibody library with the library capacity of 1.85 multiplied by 10 9 Meets the standard of a large-capacity antibody library, and can realize the continuous capacity increase of the antibody library. The invention does not need to immunize camels, has low cost and short time consumption, and can realize the screening of single-domain antibodies of various types of antigens (such as CD 20). |
| priorityDate | 2022-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 320.