http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114081658-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efa7eacd0d1fdc4690ad3b46d048eb0c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B2503-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B2503-42 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61D1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B10-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61D1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61B10-02 |
| filingDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9fafdae486a4132429a9b83938974d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeb9c7c2594ba998379b312d1e7858f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2342a3ad4635d3732712747cf13a182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a63a35227ec00e95dcecb7d7f8471c1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7dc03c805e6f2cc74cba1f62881d347 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d99c5954cf035a5f0522375009531006 |
| publicationDate | 2022-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114081658-A |
| titleOfInvention | Material taking method suitable for DRG (dorsal root ganglion) of rat |
| abstract | The invention belongs to the technical field of tissue sample acquisition, and discloses a material taking method suitable for rat dorsal root ganglion DRG, which comprises the following steps: taking dead rats to determine the position of an iliac crest, and cutting off and separating spines; putting the separated spine into hydrogen peroxide for washing and soaking for one minute; finding the L5 vertebral body from the cross section of the removed lumbar spine segment; respectively shearing off the muscles on the horizontal planes at the two sides of the vertebral body; separating the converged spinal nerves and muscle tissues in sequence, taking out the dorsal root ganglia and the central ends and the peripheral ends of the two ends of the dorsal root ganglia, and only keeping the dorsal root ganglia; putting the dorsal root ganglion into a centrifuge tube, temporarily putting into liquid nitrogen, and transferring into a refrigerator at the temperature of minus 80 ℃ for storage. The invention can quickly position the spinal ganglia and ensure that the dorsal root ganglia are not polluted by bloodstains, thereby providing high-quality tissue samples for histological and morphological experiments. The invention has the advantages of less time consumption, simple and convenient operation, more complete back root ganglion and no pollution by bloodstain. |
| priorityDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.