http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114075295-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_48ec181709fa4023ce17d589fa6b0c88 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
filingDate | 2020-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeadcf4935fbf9a5771011c63055f532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bce3ee3d89d9d788cb6d128ffad4d11b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2f99e6775a2d83d2e6520344b198063 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_694b5a15bde8f25dfc1004b722ff8452 |
publicationDate | 2022-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114075295-A |
titleOfInvention | Efficient renaturation liquid of Boc-human insulin fusion protein inclusion body and renaturation method thereof |
abstract | The invention relates to a high-efficiency renaturation solution of a Boc-human insulin fusion protein inclusion body and a renaturation method thereof. Specifically, the invention provides a method for renaturation of Boc-human insulin fusion protein, which comprises the following steps: (1) cracking the inclusion body containing the Boc-human insulin fusion protein to obtain a cracking mixed solution, and adding a denaturing solution into the cracking mixed solution to obtain a Boc-human insulin fusion protein denaturing solution; (2) and mixing the Boc-human insulin fusion protein denaturation solution with a renaturation solution and then reacting to obtain the renatured Boc-human insulin fusion protein. The method of the invention can improve the renaturation efficiency. |
priorityDate | 2020-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 248.