http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114032287-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad7bd5adfbb11470ee5f6d0d5e9faabb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 |
| filingDate | 2021-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e43cdde8fd584d96b70ac9ff9301a6d1 |
| publicationDate | 2022-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114032287-A |
| titleOfInvention | DNA methylation sequencing library and construction method and detection method thereof |
| abstract | The application discloses a DNA methylation sequencing library, a construction method and a detection method thereof. According to the method, the double-stranded DNA is not subjected to end repair when a sequencing library is established, but the double-stranded DNA is firstly denatured into the single-stranded DNA, and then the single-stranded DNA is used as a library establishing template and combined with an enzymology conversion method to establish a DNA methylation sequencing library, so that the phenomenon that a repair base is read by a sequencer due to end repair can be avoided, and the redundant phenomenon of sequencing data caused by the phenomenon is avoided. In addition, the application does not adopt unmethylated dNTPs for end repair, and does not introduce new methylation level into the end, thereby causing distortion phenomenon of the measured methylation level. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114438184-A |
| priorityDate | 2021-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.