http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114032252-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6491 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
filingDate | 2021-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114032252-B |
titleOfInvention | Expression method and application of recombinant human MMP-7 protein |
abstract | The invention belongs to the technical field of expression of recombinant proteins of mammalian cells, and particularly relates to an expression method and application of a recombinant human MMP-7 protein. According to the invention, the combination and collocation of 3 signal peptides and 3 tags are designed for the human MMP-7 recombinant protein, and finally the secretory expression of the protein is realized under the coexistence condition of the signal peptide B, the humanFc-C-6His tag, the signal peptide B and the mouseFc-C-6His tag. The signal peptide B and the humanFc-C-6His tag are combined and matched with protein with better expression quantity, MMP-7 recombinant protein is successfully obtained through WB verification and affinity purification, the expression quantity is about 24.53mg/1000mL Cells, and the transfection expression process can be optimized subsequently, so that higher expression quantity is realized. |
priorityDate | 2021-11-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 437.