http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114027189-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008
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filingDate 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-114027189-B
titleOfInvention A method for obtaining regenerated plants from mature embryos of common camellia oleifera through somatic embryogenesis
abstract The invention discloses a method for obtaining regenerated plants from mature common Camellia oleifera embryos through somatic embryogenesis, comprising: taking mature common Camellia oleifera embryos in mid-October and sterilizing them as explants; pre-cultivating the explants, and subculture Carry out differentiation culture on the differentiation medium to obtain embryogenic callus; wherein, the differentiation medium is added with 0.05~0.15mg/L 2,4-D+0.05~0.15mg/L KT; the embryogenic The callus is transferred to the embryoid body development and plant regeneration medium for regeneration culture, and the embryoid body with obvious cotyledon is obtained; the embryoid body with obvious cotyledon is transferred to the rooting medium for rooting culture, and the rooted embryoid body is formed. Whole plants, after hardening and acclimatization, are transplanted into the cultivation medium for cultivation. The differentiation rate of embryogenic callus tissue and embryoid bodies reaches over 25%, the rate of regenerated plants reaches over 40%, and complete regenerated plants are obtained within 6 months.
priorityDate 2021-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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