http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114015670-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01009 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
filingDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2023-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-114015670-B |
titleOfInvention | A kind of histidinol phosphate aminotransferase mutant 138, engineering bacteria and application |
abstract | The invention discloses a histidinol phosphate aminotransferase mutant 138, an engineering bacterium and its application, and belongs to the field of biotechnology. The histidinol phosphate aminotransferase gene is derived from Streptomyces luteus luteus strain HY61, its nucleotide sequence is shown in SEQ ID NO:1, and its amino acid sequence is shown in SEQ ID NO:2. The mutant 138 is obtained by a single mutation at the 138th position of the amino acid sequence shown in SEQ ID NO: 2, and the enzyme activity of the screened mutant is increased to 112.4% compared with the original one. The histidinol phosphate aminotransferase mutant engineering strain constructed by the present invention, combined with nitrogen protection and the use of stabilizers, effectively improves the stability of 3-indolepyruvate, and the raw materials are cheap and easy to obtain, and the production process is simple and easy. , The production cost is lower, and the high-yield green production of 3-indolepyruvate is realized. |
priorityDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 218.