http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114002440-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2021-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114002440-B |
| titleOfInvention | Enzyme-linked immunoassay kit for human phosphorylated vasodilator-stimulated protein and detection method thereof |
| abstract | The invention discloses an enzyme-linked immunoassay kit for human phosphorylated vasodilator-stimulated protein and a detection method thereof, wherein the assay kit comprises a sample pretreatment buffer solution, a sample activator containing PGE1, a sample inhibitor containing PGE1 and ADP, an ELISA plate, a VASP monoclonal antibody, a color development liquid and a stop solution; wherein the VASP monoclonal antibody is horseradish peroxidase-labeled murine monoclonal antibody, the murine monoclonal antibody heavy chain complementarity determining region comprises CDR-H1, CDR-H2 and CDR-H3 fragments, and the murine monoclonal antibody light chain complementarity determining region comprises CDR-L1, CDR-L2 and CDR-L3 fragments. The invention adopts an enzyme-linked immunosorbent assay, optimizes and improves the sequence of the monoclonal antibody, has high capture rate and good specificity of the improved monoclonal antibody, overcomes the influence of a whole blood sample on a detection result, and avoids the step of processing the sample to be detected before the previous detection. |
| priorityDate | 2021-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.