| abstract |
The invention relates to siRNA for inhibiting PCSK9 gene expression, wherein the sense strand nucleotide sequence is shown as SEQ ID NO.1, the antisense strand nucleotide sequence is shown as SEQ ID NO.3, and the siRNA generated by annealing a new nucleotide sequence formed by respectively substituting, deleting and/or adding 1-6 bases in the sense strand nucleotide sequence and the antisense strand nucleotide sequence is structurally modified by various chemical modification methods, so that the siRNA has good inhibition activity on the expression of the gene PCSK 9. The siRNA is subjected to ligand modification to form a new conjugate, and the conjugate has high inhibition activity and stability, has high liver targeting property and capacity of promoting endocytosis of cells, has excellent affinity and provides possibility for future targeted therapy. |