| abstract |
The invention provides an animal-origin-free NK cell culture medium and a culture method of NK cells, and relates to the field of cell culture. The NK cell animal-origin-free culture medium comprises an RPMI-1640 basic culture medium, a nut extracting solution, a unicellular algae extracting solution, interleukin-2, albumin and antibiotics, wherein 40-80 ml of the nut extracting solution, 20-60 ml of the unicellular algae extracting solution, 230-80 mu g of the interleukin, 35-45 g of the albumin and 8 x 10 of the antibiotics are added into each liter of the RPMI-1640 basic culture medium 5 ～10 6 And U is adopted. Through selecting nuts and algae to extract, and adding the extracting solution into a basic culture medium, the supply of trace elements is realized, and the trace elements are simply and quickly added. |