http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113970536-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-918 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-643 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113970536-B |
| titleOfInvention | A kind of rapid detection method for carbamate pesticides in vegetables |
| abstract | The invention discloses a method for rapid detection of carbamate pesticides in vegetables, which includes setting a standard concentration of carbamates in a substrate to be detected; vortexing and standing, adding ultrasonic-assisted extraction for 0.5-1 h; The detection matrix solution was added with anhydrous magnesium sulfate and sodium chloride in a mass ratio of 1:3:1, vortexed for 1 min, centrifuged for 5 min, and the centrifuged supernatant was added with anhydrous magnesium sulfate and PSA in a mass ratio of 10:1:0.2. Mix the centrifuged solution and 20% methanol solution according to the volume ratio of 1:1 to obtain a reconstituted solution; take the reconstituted solution according to the volume ratio of 10:1, add the immobilized enzyme solution according to the volume ratio of 10:1, mix by 10:1, vortex and shake, and allow to stand for reaction. The mixture was reacted with 11:10 IDA solution for 3 min, and the supernatant was collected after magnetic separation to detect fluorescence. The invention utilizes magnetic mesoporous silica nanoparticles as a carrier to covalently couple and immobilize acetylcholinesterase, thereby improving the accuracy of rapid detection of carbamate pesticides in vegetables. |
| priorityDate | 2021-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 92.