| abstract |
The invention belongs to the field of tumor biology, and relates to human tumor-associated fibroblasts and application thereof. The human tumor-associated fibroblast cells of the present invention have the following biological properties: the cells are in a long fusiform or starburst shape, the cytoplasm is rich and acidophilic, the cell nucleus is positioned in the center of a cell body, the cell nucleus is circular or oblong, the cells are arranged in a radial or bundle shape, part of the cells are disorderly arranged, the polarity disappears, and the obvious cross overlapping phenomenon exists; stable passage within ten generations; the cell expression protein alpha-SMA, FAP, E-cadherin and Vimentin are positive. The invention has the advantages that: can be cultured in vitro for a long time and keep the cell characteristics unchanged, is a powerful tool for the relevant research of the tumor-related fibroblasts, and is a powerful tool for researching the tumor-related fibroblasts mediated tumorigenesis development and the relevant molecular action mechanism, developing a tumor treatment drug target, developing a new tumor biological treatment technology and detecting the research of relevant biological engineering products. |