http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113957032-A

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filingDate 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38854a258b3ce0b1ec94b535cd2f8d64
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publicationDate 2022-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113957032-A
titleOfInvention A kind of breeding method of high ethanol tolerance strain
abstract The application relates to the field of strain selection and breeding, in particular to a method for breeding high ethanol-tolerant strains; the method includes: obtaining a fermentation strain; first screening and culturing the fermentation strain to obtain a pure strain The pure-bred bacterial strain is carried out the first cultivation, until the ethanol concentration reaches the first set concentration, obtains the fermentation mash; The ethanol is gradually added in the fermentation mash, until the ethanol concentration reaches the second set concentration, and carries out the first 2. Screening and culturing to obtain a number of high-tolerance strains; a plurality of high-tolerance strains are expanded and cultured, and then the first and second cultures are performed to obtain high-tolerance strains and ethanol-producing strains, respectively. According to the ethanol yield, determine whether the high-tolerance strain is qualified; if so, freeze the high-tolerance strain to obtain a high-ethanol-tolerant strain; through the above method, the high-ethanol-resistant strain can be accurately screened out. The ethanol-tolerant strains were obtained to obtain high ethanol-tolerant Clostridium ethanolic bacteria.
priorityDate 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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