| abstract |
The invention provides a construction method and application of Escherichia coli engineering bacteria that can completely degrade nitrobenzene, and relates to the field of genetic engineering. , nbzCa, nbzcb, nbzD, nbzE, nbzF, nbzG, nbzH, and nbzI were structurally optimized according to the codon preference of E. coli to obtain nbzAS, nbzBS, nbzCaS, nbzCbS, nbzDS, nbzES, nbzFS, nbzGS, nbzHS, and nbzIS Gene; select T7 promoter and terminator to control the expression of the optimized ten genes, and complete the splicing of the optimized ten gene prokaryotic expression units; assemble a fully degraded nitrobenzene based on two compatible plasmids Expression unit: The double plasmid with the expression unit completely degraded by nitrobenzene was transferred into E. coli with T7RNAP gene to obtain an E. coli engineering strain containing ten gene prokaryotic expression units. The engineering bacteria can completely degrade nitrobenzene with a concentration of 3 mM within 8 hours, and the engineering bacteria have the ability to degrade organic pollutants nitrobenzene. |