| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_389558a3b8150f957c96cf7125bd380a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45ed05cf60abf7607ec59f1f45c78458 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-01037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y103-03003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 |
| filingDate |
2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7941c4abf94224e0fcb11fbe7cb3ae0f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6108af16b30b54371fcb2d2b5333ce1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_832330d920ead55073be78bf33abcf36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d803195c8cc15a9494165321c4db96d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_187957e3f7aa697a4583d4a9bdaa7a24 |
| publicationDate |
2022-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113930439-A |
| titleOfInvention |
5-aminolevulinic acid producing strain and construction method thereof |
| abstract |
The invention discloses a 5-aminolevulinic acid producing strain and a construction method thereof, wherein the construction method comprises the following steps: (1) knocking out the genes of pxpR, PoxB, ptsG, sucC, sucD and pta in the bacillus subtilis to obtain bacillus subtilis engineering bacteria; (2) carrying out double enzyme digestion on the plasmid pHT43-J by using AatII and SmaI, integrating the hemO gene on the plasmid pHT43-J subjected to double enzyme digestion to obtain a recombinant plasmid pHT43-J-hemO, carrying out enzyme digestion on the recombinant plasmid pHT43-J-hemO by using Xba I and BamH I, and integrating the rhtA gene on the recombinant plasmid pHT43-J-hemO subjected to enzyme digestion to obtain a first recombinant expression vector; carrying out double enzyme digestion on the plasmid pHT01 by using BamH I and Xba I, and integrating the hemF gene into the plasmid pHT01 subjected to double enzyme digestion treatment to obtain a second recombinant expression vector; (3) and introducing the obtained first recombinant expression vector and the second recombinant expression vector into bacillus subtilis engineering bacteria to construct and obtain the 5-aminolevulinic acid producing bacteria. The production bacteria of the invention improve the yield of the 5-aminolevulinic acid. |
| priorityDate |
2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |