http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113930388-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75df1d17c60d932d89d802d7031b7bfa |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6c0e5211898ec385c47935f9d199cea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11d502219bd582d6ea0d189d3ce17b5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8ce7cea119d61ad7be9ee5971e0b12d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d36ce308f3083fdcacf8efe083006c3c |
| publicationDate | 2022-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113930388-A |
| titleOfInvention | Chemical embryotoxicity prediction model and establishment method thereof |
| abstract | A chemical embryotoxicity prediction model includes a cell species; this cell type included differentiated cardiomyocytes induced by SP3 ES cells with karyotype XX. The establishment method comprises the steps of firstly, separating and culturing mouse embryo fibroblasts; secondly, in-vitro amplification culture of mouse ES; thirdly, karyotype analysis and sex identification of the mouse ES; fourthly, maintaining and identifying mouse ES pluripotency; fifthly, inducing and differentiating the mouse ES in vitro into myocardial cells; sixthly, immunochemical detection of the myocardial specific marker protein of mouse ES induced differentiated myocardial cells; seventhly, Real-time PCR detection of the expression profile of mouse ES-induced differentiated cardiomyocytes; eighthly, selecting a mode compound; ninth, detection of cytotoxicity of model compounds; tenthly, detecting the inhibitory effect of the model compound on the ES differentiation capacity; eleventh, evaluation of model compound embryotoxicity. Filling the blank of the embryonic stem cells of the female-free mice in the EST system. |
| priorityDate | 2015-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 204.