| abstract |
The invention discloses a method for separating human urinary kallidinogenase and thrombin regulatory protein in the field of biotechnology. The method comprises the steps of separating a human urinary kallidinogenase crude product and a thrombin regulating protein crude product for the first time by adopting a hydrophobic chromatography one step, wherein a hydrophobic ligand of a hydrophobic chromatography column is one of phenyl, butyl sulfide and octyl, and a filler main body framework of the hydrophobic chromatography column is one of agarose, cellulose or a polymer. The method has the advantages of simple operation, low cost, easy industrial amplification, high yield of two separated products and obvious impurity removal effect. |