http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113899898-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d6caadbc395b3d6ec4ecb6783f1a50d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2033-184 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 |
| filingDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2196913ae97372b41ba47d04923d0202 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37e0e5aaefb85180aa570b261ac4be56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b65c4666e4f8707216f2ad064ef125a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa421cf1d6695f41c4e3c9ae70cc419a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8852a42f1cb09f13f5d64fc162663118 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e40878ebbdafe7465cf0a2368b52a9e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc9d7ce8abf500c5f0cc371d6fdad6da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f26af6f00745fb6694aef9fb7866767c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a041edafe912f3869cf5fe79de3b169 |
| publicationDate | 2022-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113899898-A |
| titleOfInvention | Carbofuran immunodetection method based on reverse fluorescence enhanced double-channel signal and antibody |
| abstract | The invention discloses a carbofuran immunodetection method based on reverse fluorescence enhanced two-channel signals, which is characterized in that a competitive immunochromatography method is utilized, a sample treatment solution and a carbofuran monoclonal antibody-dopamine gold-coated probe are mixed and incubated, then a sample pad is dripped, and whether the sample contains carbofuran or not is judged by observing the color change and the fluorescence change of a detection test strip; according to the method, the fast detection of double signals of carbofuran is realized by observing the purple red strip of dopamine-coated gold and the green fluorescent strip of quantum dots, and the lowest detection limit is 3.13 ng/mL; wherein the amino acid sequence of the variable region of the carbofuran monoclonal antibody heavy chain is shown as SEQ ID NO.6, and the amino acid sequence of the variable region of the light chain is shown as SEQ ID NO. 8; the method changes the read signal and the target concentration into a positive correlation relationship through the recovery-quenching relationship conversion of fluorescence, improves the detection sensitivity, and has the characteristics of simple operation and high field detection speed. |
| priorityDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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