http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113881630-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0781 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-17 |
| filingDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113881630-B |
| titleOfInvention | Method for culturing and separating tumor specific TIL cells |
| abstract | The invention provides a method for culturing and separating tumor-specific TIL cells, which comprises the following steps: step 1, obtaining tumor cell masses and immune cell fluid in the pleural effusion and the abdominal fluid; step 2, culturing the tumor cell mass in tumor organoid; step 3, performing amplification culture on the immune cells; step 4, processing the tumor organoids obtained by culture into single cells, and then co-culturing the single cells and the immune cells subjected to amplification culture; and 5: isolating a specific immune cell population in the co-cultured cells. The tumor organoid and the tumor-related immune cells are simultaneously isolated and cultured by using a sample from the same source, and then the tumor organoid and the immune cells are co-cultured, so that the obtained tumor-specific TIL cells can retain the biological effect of the organoid, are large in quantity, and obviously shorten the culture period. In addition, TIL cells obtained by separation and culture by the method have stronger tumor specificity, so that the tumor killing activity is stronger. |
| priorityDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.