| abstract |
The invention provides an anti-anoxic active Dendrobium officinale polysaccharide and a steam explosion preparation method and application thereof, belonging to the technical field of medicine. In the method, the raw material of Dendrobium officinale is subjected to steam explosion treatment with specific parameters, and then subjected to water extraction and alcohol precipitation extraction to remove protein and small molecular impurities, and further purifies by DEAE-Cellulose anion exchange column chromatography and Sephadex column chromatography. Collected small molecular weight (6.2-8.4 kDa) polysaccharides. The above method degrades the macromolecular polysaccharides in Dendrobium officinale into small molecular polysaccharides under the action of steam explosion, changes the chemical groups of the polysaccharides and the chemical composition of the monosaccharides, and increases the content of uronic acid in the polysaccharide components with anti-hypoxic activity, thereby increasing the content of uronic acid. The prepared polysaccharide has the activity of significantly improving the acute hypoxia tolerance of the body, and can be used for preparing medicines or functional foods for resisting acute hypoxia injury. |