| abstract |
The invention discloses a composite amplification kit for simultaneously detecting three chronic disease susceptibility genes and application thereof, wherein the kit comprises primers for amplifying SNP sites of related loci of kidney diseases, type II diabetes and cerebral hemorrhage. Compared with the prior art, the invention has the following advantages: (1) the kit improves the SNP recognition capability of the primers through primer optimization, so that the primers can effectively recognize the difference of one base, the optimal annealing temperature range is enlarged, the false positive of a detection result caused by the accuracy requirement of the annealing temperature is avoided, and the detection accuracy and reliability are improved; (2) the kit combines an ARMS technology with a capillary electrophoresis method for the first time, simultaneously detects 10 SNP sites of three chronic diseases, and is the kit with the platform for detecting the most sites of the three chronic diseases; (3) the kit is convenient to use, the operation method is simple, and the detection time is shortened. |