http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113841616-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-002
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2021-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113841616-B
titleOfInvention Gynura bicolor callus high-frequency differentiation culture medium and Gynura bicolor callus culture method
abstract The invention discloses a high-frequency differentiation method of a plant jade dew callusThe culture medium comprises inorganic salt, organic component, carbon source, additive, coagulating agent and hormone, wherein the inorganic salt contains NH 4 NO 3 、KNO 3 、CaCl 2 、KH 2 PO 4 、MgSO 4 KI, etc., organic components containing Na 2 EDTA, inositol, nicotinic acid, thiamine hydrochloride, pyridoxine hydrochloride and glycine, wherein the carbon source is sucrose, the additive is a rubicuna yunnanensis callus extract, the coagulant is agar, and the hormone is 6-BA and NAA. The culture medium can obviously improve the differentiation capacity of cells, realizes high-frequency differentiation and regeneration of callus, enables the formation of a plant with good growth state under the condition of no root system, does not need to change the type of the culture medium in the whole culture period, obviously shortens the redifferentiation time of the callus, obtains a large amount of tissue culture seedlings which are easy to survive, is not limited by seasons in the culture process, and effectively improves the propagation efficiency and economic benefit.
priorityDate 2021-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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