http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113832189-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2021-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113832189-B |
| titleOfInvention | A gRNA for knocking out IGHG region of porcine immunoglobulin heavy chain and its application |
| abstract | The invention discloses a gRNA for knocking out IGHG region of porcine immunoglobulin heavy chain and its application. A technical solution to be protected by the present invention is a method for knocking out the IGHG region of a porcine immunoglobulin heavy chain, the method comprising introducing into porcine cells to reduce or inhibit the protein activity or content of the IGHG region of the porcine immunoglobulin heavy chain constant region the substance or the substance that regulates the expression of the protein-encoding gene. The present invention utilizes the feature of many repeating sequences in the IGHG region of the porcine immunoglobulin heavy chain, and realizes multiple cleavage at multiple sites in the IGHG region by using the CRISPR-Cas9 technology of combining the four gRNAs shown in Sequence 2 in the sequence table with nucleic acid molecules , and finally achieved the simultaneous knockout of the IGHG region up to 110kb in the constant region of the immunoglobulin heavy chain in pigs, which affects the immunogenicity of the antibody, laying the foundation for the establishment of human immunoglobulin transgenic pigs. |
| priorityDate | 2021-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 221.