| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7cc315cc5a1fe3bf4c1730dd5a48c1f2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-108
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
2021-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ccbd9b073812b3eff299931625cd9271
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_894b31e2a5a14d126f8220cc6684e428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5fcbf9209b1c628e6d8378478fe3637
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e10e311bdbb3c94d8716bf30375351d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8d4713a4e966f804eb65b16f4db31e1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10612d5fe7bd9ae7ca01f9228ffa98c6 |
| publicationDate |
2021-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113832189-A |
| titleOfInvention |
A gRNA for knocking out IGHG region of porcine immunoglobulin heavy chain and its application |
| abstract |
The invention discloses a gRNA for knocking out IGHG region of porcine immunoglobulin heavy chain and its application. A technical solution to be protected by the present invention is a method for knocking out the IGHG region of a porcine immunoglobulin heavy chain, the method comprising introducing into porcine cells to reduce or inhibit the protein activity or content of the IGHG region of the porcine immunoglobulin heavy chain constant region the substance or the substance that regulates the expression of the protein-encoding gene. The present invention utilizes the characteristics of many repeating sequences in the IGHG region of the porcine immunoglobulin heavy chain, and realizes multiple cleavage at multiple sites in the IGHG region by using the CRISPR-Cas9 technology of combining the four gRNAs shown in Sequence 2 in the sequence table with nucleic acid molecules Finally, the simultaneous knockout of the IGHG region up to 110kb in the constant region of the immunoglobulin heavy chain in pigs that affects the immunogenicity of the antibody is achieved, which lays the foundation for the establishment of human immunoglobulin transgenic pigs. |
| priorityDate |
2021-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |