| abstract |
The invention discloses a dust mite microsatellite marker, which is selected from at least one of Df01, Df02, Df03, Df04, Df05, Df06, Df07, Df08, Df09, Df10, Df11 and Df12, Df01 to Df12 are DNA sequences of SEQ ID NO.01 to Df12 or complementary sequences thereof in sequence, and microsatellite repeated motifs are (CTG) in sequence 5‑8 、(ATG) 5‑7 、(ATC) 9‑12 、(GTT) 6‑7 、(ATC) 6‑8 、(TG) 6‑9 、(ATG) 5‑9 、(GT) 7‑10 、(ATG) 4‑5 、(CAA) 5‑7 、(TTG) 6‑9 And (CA) 7‑8 . The microsatellite marker and the primer thereof provided by the invention have the characteristics of stable PCR amplification, high polymorphism and the like, and can be used for carrying out genetic diversity and genetic knot of dust mitesThe structure analysis has higher application value. |