http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113801196-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eac2f8eff077c4682236f6b66083d39a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-55 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-04 |
filingDate | 2020-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62d484c4c16b4155a2ed191441c94a13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6224c5aa221e3644b36c7262db3e81a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2666cdaa1f51a811fc533d1cca47e163 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07b0f80a872040cbadb32eb455601b70 |
publicationDate | 2021-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113801196-A |
titleOfInvention | Preparation method of procapsipeptide |
abstract | The invention relates to the technical field of polypeptide preparation, in particular to a preparation method of procatide. The present invention utilizes soluble fragment peptide resins: mmsb- [ Lys (Boc)] n -introducing soluble fragments into the resin at the C-terminus of the peptide sequence, coupling each protected amino acid residue in sequence by a linker arm, then performing cyclization of two pairs of disulfide bonds in the solid phase to obtain a filtrate after cleavage of the peptide resin and NH 4 And (3) carrying out reaction on the I and DMS to break soluble fragments, precipitating by diethyl ether to obtain crude peptide, and then preparing by reversed phase chromatography to obtain refined peptide. The existence of the soluble fragment changes the peptide sequence structure during cyclization, so that the proportion of topological impurities generated after cyclization is greatly reduced, the purity of the crude peptide after cyclization is greatly improved, and simultaneously, the introduction of the C-terminal soluble fragmentThe swelling property of the peptide resin in the coupling process is enhanced to a certain extent, the coupling difficulty of the subsequent amino acid residues is reduced, and the yield of the final product is improved. |
priorityDate | 2020-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 155.