http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113785073-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
filingDate | 2020-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cebdc7ce3318bbc89059e6c610d2864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1dbb2e0108a5362fe85db44d331d06e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_047371656eb41c97b3d0e50e4add3afd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65074ef243e32a47650feddf8ebbb938 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3be1b1ac2f70e49f11f894df0c4d51bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_afa71f382b22ab854bfc6a6014879899 |
publicationDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113785073-A |
titleOfInvention | Preferential/selective amplification of RNA versus DNA targets using dITP based on strand isolation temperature |
abstract | The present disclosure describes methods for preferentially and/or selectively detecting and/or quantifying a target RNA relative to DNA in a biological or non-biological sample. The method comprises performing an amplification step, a hybridization step, and a detection step. In addition, the reaction mixture includes dntps, which include dITP. Kits, reaction mixtures and oligonucleotides (e.g., primers and probes) for preferential and/or selective amplification and detection and/or quantification of target RNA (versus DNA) in the presence of dITP are also described. These methods, kits and reaction mixtures are useful for preferentially and/or selectively detecting and/or quantifying RNA over DNA, and are particularly useful for samples containing a variety of different types of nucleic acids (e.g., DNA and RNA). |
priorityDate | 2019-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 160.