http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113774496-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb1b405433b624e4863a05e30e5f48e8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2021-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_886cc464c2066b6ec114ed9cf38c17d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_083f1c625ab321b4c1bac5c44408687a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5bb18275d91b8985f1ad25e20702234 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_559cee7a1155cb4003e6a976ea6938c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2e43865cbc6821d2539df6363a223c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_732e9f63ecd56e355a5479b00dced8e5 |
| publicationDate | 2021-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113774496-A |
| titleOfInvention | Liquid phase capture library construction method |
| abstract | The invention belongs to the technical field of molecular biology, and particularly relates to a liquid phase capture library construction method. (1) Obtaining a double-stranded DNA fragment subjected to 5' end joint connection, wherein the length of the double-stranded DNA fragment is 100-300 bp; (2) adding a probe into the system in the step (1) to capture a target area; (3) performing 3' end auxiliary connection on the captured target region DNA single strand or cDNA to extend into a DNA double strand; (4) and (4) directly amplifying the extension product obtained in the step (3) by using a universal primer to obtain a target region library. Compared with the prior art, the method omits the step of constructing a pre-library, creatively omits the linker sequence blocking oligonucleotide which is necessary to be used in the prior art, and shortens the whole library construction time to be within 6 hours. Therefore, the operation process is simpler and is not easy to make mistakes, the detection cost is greatly reduced, and the method has a good application prospect. |
| priorityDate | 2021-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.