| abstract |
The invention discloses a construction method and application of a dual miRNA-initiated master key-drug delivery system, including gold nano-cages, fluorescent chemotherapeutics and DNA bridging nano-locks. The fluorescent chemo-therapeutics are loaded in the gold nano-cages cavity. Inside, the DNA bridging nanolocks are combined with the surface of the gold nanocages to block the cavities of the gold nanocages, and the DNA bridging nanolocks are composed of SH-modified long-chain DNA S1, SH-modified long-chain DNA S2, with The long-chain DNA S3 of the composition sequence of the Mg 2+ enzyme structure and the long-chain DNA S4 with the recognition sequence of the Mg 2+ enzyme are composed. S1 and S2 are bound to the surface of the gold nanocages through gold-sulfur bonds, and S3 is complementary to the partial base of S1. , the two ends of S4 are complementary to the bases at the free ends of S1 and S2, respectively. It utilizes the Mg 2+ -dependent DNase cyclic shearing reaction of endogenous substances in cells, and performs a master key to open multiple locks. The drug can be targeted and enhanced in cancer cells to achieve the signal amplification diagnosis and treatment function of the probe. |