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filingDate 2021-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b67c0e20ca0b86ec7734a89653269fc5
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publicationDate 2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113759119-A
titleOfInvention Kit for fluorescence immunoassay rapid detection of malachite green carbon quantum dots in aquatic products and detection method thereof
abstract The invention discloses a kit for rapidly detecting malachite green carbon quantum dots in aquatic products by fluorescence immunoassay and a detection method thereof, belonging to the field of fluorescence immunoassay. The kit consists of a reaction cup and a reagent; wherein the reaction cup is coated with a coating antigen, and the reagent is marked with an antibody working solution by horseradish peroxidase; malachite green series standard solution; 20 times of concentrated phosphate washing solution; concentrating the phosphate complex solution by 10 times; 10 is composed of a concentrated sample extracting solution 1, a carbon quantum dot fluorescence reaction solution A and a carbon quantum dot fluorescence reaction solution B. The carbon quantum dots are used as a fluorescent substrate, and a malachite green fluorescence quenching immunoassay method is established. The detection time of the kit is 30min, the detection limit is 0.032ng/mL, the intra-batch variation coefficient is less than 8%, the inter-batch variation coefficient is less than 15%, and the recovery rate of the aquatic product sample is 90% +/-30%. The fluorescence immunoassay rapid detection kit has the advantages of high specificity, good sensitivity, simple and convenient operation and capability of realizing quantitative detection.
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