http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113755527-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70596 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-0405 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2021-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113755527-B |
| titleOfInvention | Plasmid vector for simultaneously expressing PLAUR and GPLD1 genes and construction method thereof |
| abstract | The invention discloses a plasmid vector for simultaneously expressing PLAUR and GPLD1 genes and a preparation method thereof. In the construction process of the plasmid vector, a section of DNA containing the enzyme cutting sites of Nhe I-Age I-Sbf I-Ecor I-Nsi I-Mlu I-avrli-BamHI is designed, the fragments are recovered after the Nhe I and BamHI are subjected to double enzyme cutting, and the recovered fragments are cloned to a pCW-Cas9-Blast vector subjected to double enzyme cutting through the same enzyme cutting sites to obtain a new cloning vector pCW-DNA-Blast. And amplifying to obtain the PLAUR and GPLD1 gene segments with the enzyme cutting sites, performing the same enzyme cutting treatment on the segments and the pCW-DNA-Blast, and connecting the segments and the pCW-DNA-Blast to obtain the DNA fragment. The invention firstly expresses the PLAUR gene and the GPLD1 gene simultaneously through a plasmid vector, thereby obtaining a large amount of PLUAR protein in the cell culture supernatant. |
| priorityDate | 2021-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.