http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113755483-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66bc142b3309f47d4ec4ec63f909b6d1 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-02002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01015 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 |
| filingDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_934d77836e40cda8ae45202c98c151e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_099328f0ceaf0b005628226b28f54c79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d367879f612bdee9ac205ab2ec3c2343 |
| publicationDate | 2021-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113755483-A |
| titleOfInvention | Mutagenesis method of high-yielding pectinase enzyme activity strain and optimization of solid-state fermentation conditions |
| abstract | The invention discloses a mutagenesis method of a high-yielding pectinase enzyme activity strain and the optimization of solid-state fermentation conditions. The ethyl methanesulfonate (EMS) chemical mutagenesis method is used to mutate the strain, and the mutagenesis method is characterized in that: the mutagenesis method comprises the following steps: The following steps: after a certain amount of spore suspension is mixed and reacted with EMS for a period of time, the reaction is terminated with NaS 2 O 3 , and a certain amount of treatment solution is diluted and spread on a plate for cultivation; the induction of the high-yielding pectinase enzyme activity strain By means of the modified method and the optimization of solid-state fermentation conditions, an enzyme-producing strain with an enzyme activity of up to 19,000 U/g was successfully screened under the condition of a lethality rate of 85.8%. The optimal medium components and growth conditions of the strain were determined through subsequent optimization experiments: soybean meal 20g, initial water content 68%, glucose content 3%, peptone content 1%, CaCl 2 0.04%, FeCl 3 0.04%, inoculum size 4 %, the culture temperature is 38°C, and the enzyme activity is as high as 2200U/g after 5 days of culture, which greatly improves the enzyme-producing ability of the strain and achieves the ideal target. |
| priorityDate | 2021-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.