http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113755339-B

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filingDate 2021-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113755339-B
titleOfInvention Phenanthrene degrading fungus in petroleum-polluted soil and preparation and application of microbial inoculum thereof
abstract The invention discloses a phenanthrene degrading fungus in petroleum-polluted soil, and preparation and application of a microbial inoculum thereof. Arthrinium gutiae LJD-2 with the deposit number: GDMCC No: 61798. the invention obtains a degrading fungus strain LJD-2 taking phenanthrene as a carbon source by domesticating and separating oil contaminated soil of Shandong, identifies the strain as Arthrium gutiae LJD-2 according to the strain morphology, physiological characteristics, ITS gene sequencing analysis and phylogenetic analysis, and has the optimal growth environmental conditions as follows: the temperature is 28 ℃, the pH value is 6, and sodium chloride is not added; ITS gene sequencing analysis of the strain shows that the strain closest to LJD-2 is Arthrinium gutiae CBS 135835 (the similarity is 98.6%). LJD-2 can use phenanthrene as carbon source at an initial concentration of 50 mg.L ‑1 After the inorganic salt culture solution is cultured for 7 days, the degradation rate of the pure bacteria can reach more than 55 percent. After the strain is prepared into the solid microbial inoculum, the degradation rate of phenanthrene can be increased to more than 70%, so that the strain has better application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.
priorityDate 2021-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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