| abstract |
The present invention belongs to the technical field of characterization of target analyte properties, and specifically provides a mutant of a porin monomer, a protein pore comprising the same, and an application thereof for detecting a target analyte, wherein the mutant of the porin monomer is provided. The amino acids of include the sequence set forth in SEQ ID NO: 1 or a sequence having at least 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60% or 50% identity thereto, And the amino acids of the mutants of the porin monomers include mutations at one or more positions corresponding to Q68, N72, and F73 of SEQ ID NO:1. |