abstract |
The invention discloses a bitter-free soybean polypeptide with blood fat reducing activity and a preparation method thereof, wherein isolated soybean protein and water are stirred, and then are uniformly stirred at 80-95 ℃, cooled to room temperature and adjusted to pH value of 6-8, a compound protease preparation and an internal cutting type protease preparation are added, and the mixture is stirred in a water bath at 45-60 ℃ for 5-8 hours to inactivate enzyme; centrifuging, collecting supernatant, and vacuum freeze drying to obtain soybean polypeptide; the soybean polypeptide prepared by the invention has no bitter taste, good flavor, excellent solubility and remarkable blood fat reducing activity, the components with the molecular weight less than 1000Da can reach 65-72 percent, and the soybean polypeptide is mainly micromolecular peptide and has great development and application values in the fields of general foods, functional foods, medicines and the like. |