http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113736922-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2021-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113736922-B |
| titleOfInvention | A kind of enterovirus universal nucleic acid detection kit and using method |
| abstract | A universal nucleic acid detection kit for enteroviruses and a method of use of the present invention comprise an enterovirus reaction solution, a positive control solution and a negative control solution. Among them, the enterovirus reaction solution contains fluorescently labeled probes, primers, enzyme mixtures, enhancers, freeze-drying protection agents, 10xbuffer, nucleotide mixtures, etc., which can detect whether or not enteroviruses are infected in the same reaction system. The entire process of sample collection, nucleic acid extraction, amplification and detection is monitored through endogenous internal controls to avoid misjudgments caused by false negative results. Probes are oligonucleotides that include a fluorescent reporter group and a quencher group. When the probe is intact, the quencher group is sterically close to the reporter group to inhibit the reporter group from emitting fluorescence. When the primer is extended, the probe bound to the template is cut by Taq enzyme (5'→3' exonuclease activity), the reporter group is separated from the quencher group, and a fluorescent signal is generated, so as to realize the detection of enterovirus at the nucleic acid level. detection on. |
| priorityDate | 2020-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 131.