abstract |
The invention discloses a CRISPR-Cas12a-based field rapid detection method for yam virus, which belongs to the field of biotechnology. The main points of the technical solution of the present invention are as follows: rough RNA extraction is performed on the yam tissue, RT-RPA technology is used to amplify the yam, and finally the amplified product is added to the CRISPR-Cas12a reaction system, and the detection result can be determined according to the color change of the reaction system. , the visual detection of yam virus. The invention does not rely on thermal cyclers such as PCR instruments, has the advantages of simple operation, rapid detection, visualization of results and the like, and provides a new method for field visualization detection of yam virus. |