| abstract |
The invention provides a method and kit for inducing corneal limbal stem cells in vitro. The provided method includes (1) first culturing pluripotent stem cells with a first inducer to obtain surface ectoderm cells, the first inducer comprising a first small molecule inhibitor and a first protein, and the first small molecule inhibitor Including a TGF-beta/Smad inhibitor, and the first protein includes bFGF or BMP4; (2) using a second inducer to perform a second culture on the surface ectoderm cells to obtain limbal stem cells, and the second inducer includes a second protein , the second protein includes BMP4. The provided kit includes small molecule inhibitor, protein and medium. Small molecule inhibitor includes SB505124 or SB431542; protein includes bFGF or BMP4. Using the method or the kit, the limbal stem cells can be quickly obtained, and the stability is good. |