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filingDate 2021-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e6cb70ab109530d2f4cf12b476004bc
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publicationDate 2021-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113721024-A
titleOfInvention Fluorescence immunoassay rapid detection kit and detection method for enrofloxacin carbon quantum dots in animal derived food
abstract The invention discloses a rapid fluorescence immunoassay kit and a rapid fluorescence immunoassay method for enrofloxacin carbon quantum dots in animal derived food, and belongs to the field of fluorescence immunoassay. The kit consists of a reaction cup and a reagent; wherein the reaction cup is coated with a coating antigen, and the reagent is marked with an antibody working solution by horseradish peroxidase; enrofloxacin series standard solutions; 20 times of concentrated phosphate washing solution; concentrating the phosphate complex solution by 10 times; the carbon quantum dot fluorescence reaction solution A and the carbon quantum dot fluorescence reaction solution B. The carbon quantum dot is used as a fluorescent substrate, and the enrofloxacin fluorescence quenching immunoassay method is established. The detection time of the kit is 30min, the detection limit is 0.3ng/mL, the intra-batch variation coefficient is less than 8%, the inter-batch variation coefficient is less than 15%, and the recovery rate of the aquatic product sample is 90% +/-30%. The fluorescence immunoassay rapid detection kit has the advantages of high specificity, good sensitivity, simple and convenient operation and capability of realizing quantitative detection.
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