| abstract |
The invention provides an engineered human umbilical cord mesenchymal stem cell source exosome with miRNA13896 overexpression, a preparation method and application thereof, and relates to the technical field of biological medicines. The exosome hucMSC-Ex in the supernatant of the human umbilical cord mesenchymal stem cells is separated and obtained by adopting a 30% sucrose density gradient centrifugation method, and can be stored in an ultra-low temperature refrigerator at minus 80 ℃ for a long time and keep the biological activity; the hucMSC-Ex is used as a nano-drug delivery carrier, the miRNA13896 is loaded to form the miRNA13896 over-expressed engineered human umbilical cord mesenchymal stem cell-derived exosome, the engineered human umbilical cord mesenchymal stem cell-derived exosome can be efficiently taken by tumor cells, actively targets the tumor part, inhibits the proliferation, migration and self-renewal of gastric cancer cells in vivo and in vitro, can be combined with other anti-cancer promoting drugs or materials to enhance the anti-tumor inhibition effect of the anti-cancer cells, and therefore, can be used for preparing drugs for preventing and/or treating gastric cancer. |