http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113717914-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2021-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113717914-B |
| titleOfInvention | High efficient homologous recombination engineering strain of Amycobacter, its construction method and application |
| abstract | High-efficiency homologous recombination engineering strain of Amycolatopsis, its construction method and application. The invention provides the application of ku1 and/or ku2 gene deletion in improving the efficiency of homologous recombination of Amycolatopsis sp. The engineering strain of Amycobacter lacks the ku1 gene and/or the ku2 gene of Amycolatopsis, and the invention also provides a construction method and application of the strain. In the present invention, it is verified through experiments that the deletion of the ku1 gene, the ku2 gene or the simultaneous deletion of the ku1 gene and the ku2 gene can significantly improve the homologous recombination efficiency of Amycolatopsis. Among them, the homologous recombination efficiency of the engineered strain with deletion of ku1 gene reaches 95%, which has the most prominent effect. |
| priorityDate | 2021-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.