| abstract |
The invention discloses a preparation method for cosmetic umbilical cord mesenchymal stem cell extract, and relates to the field of cell extraction. The preparation method for cosmetic umbilical cord mesenchymal stem cell extract includes: separation, preparation and identification of umbilical cord mesenchymal stem cells, acquisition of umbilical cord mesenchymal stem cell culture supernatant, and separation and purification of umbilical cord mesenchymal stem cell extract. The preparation method for cosmetic umbilical cord mesenchymal stem cell extract adopts a professional cell culture flask for adherent cells, which can stabilize the cell proliferation rate, improve the efficiency of collecting supernatant, cultivate in a hypoxic environment, and simulate the in vivo environment of stem cells. Stimulate the secretion of a large amount of cytokines, the repeated freezing and thawing of the cells makes the cells broken, and a large amount of cytokines can also be secreted. The sample source is single, and the protein concentration is determined by ELISA method to ensure the uniformity of batches of products, which is conducive to the follow-up of products. application. |