| abstract |
The invention relates to the technical field of vascular endothelial growth factor detection, and discloses a raw material preparation and detection method of a vascular endothelial growth factor detection kit, which comprises the steps of preparing raw materials, wherein the raw material preparation comprises an antibody-coated magnetic bead working solution, a VEGF-labeled antibody, a VEGF calibrator, a VEGF quality control product and a sample diluent; the antibody-coated magnetic bead working solution is a magnetic microsphere suspension coated with a humanized VEGF antibody Fab segment, and the buffer system is TBS-T (20-200mM Tris, 0-1M NaCl, 0.0-1% BSA, 0.1-0.5% Tween-20, 0.1-1% Proclin-300, pH 6.5-9.0). The luminescent marker of choice in the present invention is the acridinium salt NSP-SA-NHS. So that luminescence detection is performed in a homogeneous liquid and the reaction time is shortened. Meanwhile, the bond level of C-N is larger than that of C-O, and the stability of the conjugate of the acridinium salt NSP-SA-NHS and the protein is higher. Based on the use of NSP-SA-NHS, the sensitivity, detection range and storage time of the detection system are improved, and the reaction time is shortened. |