http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113699273-A

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filingDate 2021-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53574ffb4c919e1e3b3df61bbfc82e64
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publicationDate 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113699273-A
titleOfInvention Combination of SNP loci for detection of tomato root-knot nematode resistance and its application
abstract The invention relates to the field of plant biotechnology, in particular to a combination of SNP sites for detecting resistance to tomato root-knot nematodes and applications thereof. Based on this, the present invention develops a primer combination, a kit and a detection method that can quickly, intuitively and effectively identify the genotype state of the target SNP site. The application of the invention can realize the rapid, accurate and high-throughput detection of the haplotype of the Mi-1 segment of the tomato root-knot nematode resistance gene, and has the advantages of simple operation, low cost, automation, high throughput efficiency, stable labeling and safety. Non-toxic and harmless, it can quickly, accurately and high-throughput tomato root-knot nematode resistance identification in tomato seedling stage, reduce the workload of manual inoculation identification and field transplanting, improve breeding efficiency, reduce breeding costs, and accelerate breeding. It is very suitable for modern commercial breeding applications and large-scale genetic improvement studies.
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