| abstract |
The invention discloses a complete genome typing method based on DNA methylation locus genotype, which comprises the following steps: step one, collecting a sample; step two, establishing a DNA library; thirdly, sequencing the library; identifying sites; step five, genotyping; in the second step, the MBD treatment combined with the methylation sensitive restriction enzyme means that the endonuclease of a region not to be detected and the methylation sensitive restriction enzyme digest DNA fragments at the same time, and then the fragments containing the methylation region are captured through an MBD column and retained; the invention can judge the change of DNA methylation state under different growth states; the invention adopts two different methods for treating DNA, thereby improving the genotyping accuracy. |