http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113699107-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2318 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0646 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
filingDate | 2021-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113699107-B |
titleOfInvention | Peripheral blood NKT cell culture solution and culture method |
abstract | The invention discloses a peripheral blood NKT cell culture solution and a culture method, wherein the culture method of NKT cells comprises the following steps: adding peripheral blood mononuclear cells into a lymphocyte serum-free culture medium containing autologous serum of cell factors to culture for about 14 days; during the culture period, a culture solution containing the cell factors is required to be added every 2-3 days; after the culture is finished, performing centrifugal treatment and washing by 0.9% sodium chloride solution to obtain peripheral blood NKT cells; human serum albumin was added to peripheral blood NKT cells, and the volume was fixed with 0.9% sodium chloride solution to obtain peripheral blood NKT cells. According to the culture method, the peripheral blood single check has no immune rejection reaction to the autologous serum, the cells are purer, and the safety is higher; NKT cells grow fast and have high amplification rate; NKT cells have high killing activity and stronger tumor cell killing effect. |
priorityDate | 2021-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 162.