http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113686798-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-0112 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2021-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113686798-B |
titleOfInvention | Alpha 1 microglobulin trace detection method |
abstract | The invention discloses a trace detection method of alpha 1 microglobulin, which relates to the technical field of biological detection methods and comprises the following steps: mixing the reagent a with the sample, adding the reagent b, mixing and incubating; adding a reagent c, mixing, and reading an absorbance value; after timing reaction, reading the absorbance value again; calculating the difference value of the absorbance values twice, making a regression equation function curve of the difference value to the concentration of the alpha 1 microglobulin, and substituting the difference value of the sample to be measured into the function curve to calculate the concentration of the alpha 1 microglobulin; the reagent a comprises polyethylene glycol and ethylene diamine tetraacetic acid zinc sodium; the reagent b comprises polyvinylpyrrolidone and sodium chloride; the reagent c comprises polystyrene nanoparticles only marked with alpha 1 microglobulin monoclonal antibodies and polystyrene nanoparticles only marked with alpha 1 microglobulin polyclonal antibodies. The method effectively reduces the loss of the alpha 1 microglobulin in the sample in the detection process, reduces the detection error and improves the sensitivity by using a specific reagent and adjusting the proportion. |
priorityDate | 2021-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.