http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113684314-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c5592cf9b78763718353333e865e26ea |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c20b0d86946d46cfeea5576d4101782a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ae526e86ad9351abe7b3258db79d57e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da03d442c4f2c43d2e285ae9b5b0fb2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a879acfa71e215f1ef82de14e7eb79c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da3a0784eca0268d0de0a216a623cf80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a1c9d1337d14cb317a87c87dff55a52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ea515e0ec20c02d53bb189225e64ac6 |
| publicationDate | 2021-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113684314-A |
| titleOfInvention | A kit and detection method for detecting RBSDV virus based on CRISPR-Cas system |
| abstract | The invention discloses a kit and a detection method for detecting RBSDV virus based on a CRISPR-Cas system, belonging to the technical field of molecular detection. The invention discloses a detection method for detecting RBSDV virus based on CRISPR-Cas system, including rapid preparation of virus-bearing samples, establishment of a reverse transcription-isothermal amplification and amplification system, and a nucleic acid detection system based on four CRISPR-Cas systems , the collection of fluorescent signals and the visualization of the results presented in four main steps. The method of the invention can not only detect a single point of RBSDV, but also can perform simultaneous detection of two points in one reaction, and the detection result can be read visually by the naked eye; the detection method can be directly applied to the body of the field BPH population. The real-time monitoring of the virus-carrying rate has high application value for the prevention and control of RBSDV virus transmission. |
| priorityDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.